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A study by Cota et al (112) used antibodies to localize mTOR and showed that mTOR signalling plays a role in mechanisms in the brain that are associated with nutrient availability – the administration of leucine increased hypothalamic mTOR signalling and decreased food intake and body weight in the rats

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A lower level of enzyme activity in the erythrocytes of genetically deficient individuals might be due to a normal rate of synthesis of an enzyme of low catalytic efficiency, a decreased rate of synthesis of a normally active enzyme, an increased lability of the variant enzyme or a combined mechanism